Anticorps Recombinant de lapin anti-CD3

CD3 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

OKT3

N° de cat : 65504-1-RR

Synonymes

CD3E, T3E, TCRE

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Galerie de données de validation

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  FC experiment of human PBMCs using 65504-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-RR, Clone:OKT3) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65504-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-RR, Clone:OKT3) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65504-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 Rabbit Recombinant Antibody (65504-1-RR, Clone:OKT3) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2ug Anti-Human CD3 Mouse Monoclonal Antibdody (Clone:OKT3) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65504-1-RR cible CD3 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Human T cells
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3E
• Identification du gène (NCBI): 916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).