Anticorps Monoclonal anti-CD28
CD28 Monoclonal Antibody for FC
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
Humain
Applications
FC
Conjugaison
FITC Plus Fluorescent Dye
CloneNo.
CD28.2
N° de cat : FITC-65099
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en cytométrie | CMSP humaines, |
Dilution recommandée
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
FITC-65099 cible CD28 dans les applications de FC et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Hôte / Isotype | Mouse / IgG1, kappa |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | s/o |
Nom complet | CD28 molecule |
Masse moléculaire calculée | 220 aa, 25 kDa |
Numéro d’acquisition GenBank | BC093698 |
Symbole du gène | CD28 |
Identification du gène (NCBI) | 940 |
Conjugaison | FITC Plus Fluorescent Dye |
Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité |
Tampon de stockage | PBS with 0.09% sodium azide and 0.5% BSA. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).
Protocole
Product Specific Protocols | |
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FC protocol for FITC Plus CD28 antibody FITC-65099 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |