Anticorps Monoclonal anti-CD28

CD28 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

CD28.2

N° de cat : CL555-65099

Synonymes

CD28, CD28 molecule, Tp44

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Galerie de données de validation

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  FC experiment of human PBMCs using CL555-65099

  1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD28 (CL555-65099, Clone:CD28.2) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using CL555-65099

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD28 (CL555-65099, Clone:CD28.2) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65099 cible CD28 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD28 molecule
• Masse moléculaire calculée: 220 aa, 25 kDa
• Numéro d’acquisition GenBank: BC093698
• Symbole du gène: CD28
• Identification du gène (NCBI): 940
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).