Anticorps Monoclonal anti-CD28

CD28 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IHC, FC, Cell treatment

Conjugaison

Non conjugué

Publications

3

CloneNo.

CD28.2

N° de cat : 65099-1-Ig

Synonymes

CD28, CD28 molecule, Tp44

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Galerie de données de validation

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  FC experiment of human PBMCs using 65099-1-Ig

  1X10^6 human PBMCs were surface co-stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65099-1-Ig

  1X10^6 human PBMCs were surface stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

65099-1-Ig cible CD28 dans les applications de IHC, FC, Cell treatment et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD28 molecule
• Masse moléculaire calculée: 220 aa, 25 kDa
• Numéro d’acquisition GenBank: BC093698
• Symbole du gène: CD28
• Identification du gène (NCBI): 940
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

Publications

Species	Application	Title
human	IHC	Cancer Cell Int; Comprehensive analysis of prognostic immune-related genes and drug sensitivity in cervical cancer.; Authors - Ya-Nan Pi
human		J Transl Med; Scoring model based on the signature of non-m6A-related neoantigen-coding lncRNAs assists in immune microenvironment analysis and TCR-neoantigen pair selection in gliomas; Authors - Wenbo Zhao
human	Cell treatment	Aging (Albany NY); An integrated bioinformatic investigation of focal adhesion-related genes in glioma followed by preliminary validation of COL1A2 in tumorigenesis; Authors - Guojun Yao