Anticorps Monoclonal anti-PD-1/CD279

PD-1/CD279 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

RMP1-30

N° de cat : CL647-65142

Synonymes

CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1

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Galerie de données de validation

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  FC experiment of BALB/c mouse splenocytes using CL647-65142

  1X10^6 Con-A stimulated BALB/c mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse PD-1/CD279 (CL647-65142, Clone: RMP1-30) or isotype control antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Con-A stimulated BALB/c mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65142 cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules BHK transfectées par PD-1 de souris
• Nom complet: programmed cell death 1
• Numéro d’acquisition GenBank: BC119179
• Symbole du gène: Pdcd1
• Identification du gène (NCBI): 18566
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).