Anticorps Monoclonal anti-PD-1/CD279

PD-1/CD279 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain, primates non humains

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

EH12.2H7

N° de cat : PE-65168

Synonymes

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Galerie de données de validation

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  FC experiment of human PBMCs using PE-65168

  1X10^6 PHA treated (3d) human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul PE Anti-Human PD-1/CD279 (PE-65168, Clone:EH12.2H7) (right) or Mouse IgG1 Isotype Control (left). 1X10^6 untreated human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul PE Anti-Human PD-1/CD279 (PE-65168, Clone:EH12.2H7) (center). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PE-65168

  1X10^6 PHA treated (3d) human PBMCs were surface stained with 5 ul PE Anti-Human PD-1/CD279 (PE-65168, Clone:EH12.2H7) (red) or Mouse IgG1 Isotype Control (blue). 1X10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human PD-1/CD279 (PE-65168, Clone:EH12.2H7) (black dashed). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65168 cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains

• Réactivité: Humain, primates non humains
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: programmed cell death 1
• Masse moléculaire calculée: 288 aa, 32 kDa
• Numéro d’acquisition GenBank: BC074740
• Symbole du gène: PDCD1
• Identification du gène (NCBI): 5133
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à la R-phycoérithrine (PE) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).