Anticorps Monoclonal anti-PD-1/CD279
PD-1/CD279 Monoclonal Antibody for FC
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain
Applications
FC
Conjugaison
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
J110
N° de cat : CL647-65119
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en cytométrie | lymphocytes de sang périphérique humain, |
Dilution recommandée
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL647-65119 cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | s/o |
Nom complet | programmed cell death 1 |
Masse moléculaire calculée | 288 aa, 32 kDa |
Numéro d’acquisition GenBank | BC074740 |
Symbole du gène | PDCD1 |
Identification du gène (NCBI) | 5133 |
Conjugaison | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité |
Tampon de stockage | PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).
Protocole
Product Specific Protocols | |
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FC protocol for CL Plus 647 PD-1/CD279 antibody CL647-65119 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |