Anticorps Monoclonal anti-CD274 (PD-L1, B7-H1)

CD274 (PD-L1, B7-H1) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b

Réactivité testée

souris et plus (1)

Applications

FC

Conjugaison

PE Fluorescent Dye

Publications

2

CloneNo.

10F.9G2

N° de cat : PE-65073

Synonymes

B7 H1, Cd274, CD274 antigen, PD L1, Pdcd1l1, Pdcd1lg1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-65073

  1X10^6 mouse splenocytes cells were stained with 0.20ug CD274 (PD-L1, B7-H1) antibody (PE-65073, red) and control antibody (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

PE-65073 cible CD274 (PD-L1, B7-H1) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Rat / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD274 antigen
• Numéro d’acquisition GenBank: BC066841
• Symbole du gène: Cd274
• Identification du gène (NCBI): 60533
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C. Éviter toute exposition à la lumière.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publications

Species	Application	Title
human	FC	Biochem Pharmacol; Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT; Authors - Xing Chang
human,mouse	FC	Oncoimmunology; FXR agonist GW4064 enhances anti-PD-L1 immunotherapy in colorectal cancer; Authors - Lu Lu