Anticorps Monoclonal anti-CD25

CD25 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, lambda

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications

3

CloneNo.

PC61.5

N° de cat : APC-65137

Synonymes

CD25, Il2r, Il2ra, Ly 43

---

Galerie de données de validation

• 

  FC experiment of BALB/C mouse splenocytes using APC-65137

  1X10^6 untreated or anti-CD3/CD28 treated (2 days) mouse splenocytes were co-surface stained with FITC Plus Anti-Mouse CD4 (GK1.5) and 0.25 ug APC Anti-Mouse CD25 (APC-65137, Clone:PC61.5) or 0.25 ug APC Rat IgG1 Isotype Control (HRPN). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated 2days mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 3 publications below

Informations sur le produit

APC-65137 cible CD25 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG1, lambda
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: interleukin 2 receptor, alpha chain
• Numéro d’acquisition GenBank: BC114437
• Symbole du gène: Il2ra
• Identification du gène (NCBI): 16184
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.1% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Publications

Species	Application	Title
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
mouse	FC	Acta Biomater; Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment; Authors - Zhuo Cheng
mouse	FC	J Neuroinflammation; 27-hydroxycholesterol causes cognitive deficits by disturbing Th17/Treg balance and the related immune responses in mild cognitive impairment patients and C57BL/6J mice; Authors - Tao Wang