Anticorps Monoclonal anti-CD25
CD25 Monoclonal Antibody for FC
Hôte / Isotype
Rat / IgG1, lambda
Réactivité testée
souris
Applications
FC
Conjugaison
Atlantic Blue™ Fluorescent Dye
CloneNo.
PC61.5
N° de cat : AB-65137
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en cytométrie | plate-bound Anti-CD3 and Anti-CD28 stimulated mouse splenocytes, |
Dilution recommandée
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
AB-65137 cible CD25 dans les applications de FC et montre une réactivité avec des échantillons souris
Réactivité | souris |
Hôte / Isotype | Rat / IgG1, lambda |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | s/o |
Nom complet | interleukin 2 receptor, alpha chain |
Numéro d’acquisition GenBank | BC114437 |
Symbole du gène | Il2ra |
Identification du gène (NCBI) | 16184 |
Conjugaison | Atlantic Blue™ Fluorescent Dye |
Excitation/Emission maxima wavelengths | 404 nm / 458 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité |
Tampon de stockage | PBS with 0.09% sodium azide. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).
Protocole
Product Specific Protocols | |
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FC protocol for Atlantic Blue™ CD25 antibody AB-65137 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |