Anticorps Monoclonal anti-CD244

CD244 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2B4.69

N° de cat : CL647-65270

Synonymes

2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4

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Galerie de données de validation

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  FC experiment of human PBMCs using CL647-65270

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD56 and 5 ul CoraLite® Plus 647 Anti-Human CD244 (CL647-65270, Clone:2B4.69), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65270

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD244 (CL647-65270, Clone:2B4.69), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65270 cible CD244 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD244 molecule, natural killer cell receptor 2B4
• Numéro d’acquisition GenBank: BC028073
• Symbole du gène: CD244
• Identification du gène (NCBI): 51744
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).