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Anticorps Monoclonal anti-CD23

CD23 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4C7C9

N° de cat : CL594-60208

Synonymes

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human tonsillitis using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-60208 (CD23 antibody) at dilution of 1:50 .

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human tonsillitis using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-60208 (CD23 antibody) at dilution of 1:50 .

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human lymphoma using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CoraLite®594 CD23 antibody (CL594-60208, Clone: 4C7C9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    IF Staining of human lymphoma using CL594-60208

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CoraLite®594 CD23 antibody (CL594-60208, Clone: 4C7C9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U-937 cells using CoraLite®594-conjugated CD23 Monoclonal antibody (CL594-60208)

    FC experiment of U-937 using CL594-60208

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD23 (CL594-60208, Clone:4C7C9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFtissu d'amygdalite humain, tissu de lymphome humain

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60208 cible CD23 dans les applications de IF et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène CD23 Protéine recombinante Ag0425
Nom complet Fc fragment of IgE, low affinity II, receptor for (CD23)
Masse moléculaire calculée 321 aa, 36 kDa
Numéro d’acquisition GenBankBC064417
Symbole du gène FCER2
Identification du gène (NCBI) 2208
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocole

Product Specific Protocols
IF protocol for CL594 CD23 antibody CL594-60208Download protocol
Standard Protocols
Click here to view our Standard Protocols