Anticorps Monoclonal anti-CD226

CD226 Monoclonal Antibody for FC, ELISA

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC, ELISA

Conjugaison

Non conjugué

CloneNo.

11A8

N° de cat : 65247-1-Ig

Synonymes

CD226, CD226 antigen, CD226 molecule, DNAM 1, DNAM1, DNAX accessory molecule 1, PTA1, TLiSA1

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 65247-1-Ig

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 0.2 ug Anti-Human CD226 (65247-1-Ig, Clone:11A8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65247-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 (65247-1-Ig, Clone:11A8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65247-1-Ig cible CD226 dans les applications de FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules NK humaines
• Nom complet: CD226 molecule
• Masse moléculaire calculée: 336 aa, 39 kDa
• Numéro d’acquisition GenBank: BC074787
• Symbole du gène: CD226
• Identification du gène (NCBI): 10666
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).