Anticorps Monoclonal anti-CD223

CD223 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

C9B7W

N° de cat : CL488-65098

Synonymes

CD223, LAG 3, Lag3, Ly66, lymphocyte activation gene 3

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Galerie de données de validation

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  FC experiment of BALB/c mouse splenocytes using CL488-65098

  1X10^6 Con A-stimulated (3 ug/ml Con A, day-3) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 5 ul CoraLite® Plus 488 Anti-Mouse CD223 (CL488-65098, Clone: C9B7W). Cells were not fixed.

• 

  FC experiment of BALB/c mouse splenocytes using CL488-65098

  1X10^6 Con A-stimulated (3 ug/ml Con A, day-3) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-Rat IgG1 isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: BALB/c mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65098 cible CD223 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine de fusion CD223-Ig murine
• Nom complet: lymphocyte-activation gene 3
• Numéro d’acquisition GenBank: BC120591
• Symbole du gène: Lag3
• Identification du gène (NCBI): 16768
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).