Anticorps Monoclonal anti-CD19

CD19 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

SJ25C1

N° de cat : PE-65145

Synonymes

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using PE-65145

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug PE-anti-human CD19 (PE-65145, clone SJ25C1) (red) or 0.25 ug PE-mouse IgG1 isotype control (blue). Cells were not fixed.

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  FC experiment of human peripheral blood lymphocytes using PE-65145

  0.1 mL human whole blood cells were surface stained with 0.25 ug APC-anti-human CD3 (APC-65151, clone UCHT1) and 0.25 ug PE-anti-human CD19 (PE-65145, clone SJ25C1). Cells were then treated with red blood cell lysis buffer and were gated for lymphocytes for analysis of CD3 and CD19 staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65145 cible CD19 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NALM1 + NALM16 cells
• Nom complet: CD19 molecule
• Masse moléculaire calculée: 556 aa, 61 kDa
• Numéro d’acquisition GenBank: BC006338
• Symbole du gène: CD19
• Identification du gène (NCBI): 930
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).