Anticorps Monoclonal anti-CD19

CD19 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / Mouse IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

HIB19

N° de cat : CL647-65110

Synonymes

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using CL647-65110

  1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®488-conjugated Anti-Human CD3 (CL488-65151, Clone: UCHT1) and 5 ul CoraLite® Plus 647-conjugated Anti-Human CD19 (CL647-65110, Clone: HIB19). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL647-65110

  1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®488-conjugated Anti-Human CD3 (CL488-65151, Clone: UCHT1) and CoraLite® Plus 647-conjugated Mouse IgG1 isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65110 cible CD19 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / Mouse IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD19 molecule
• Masse moléculaire calculée: 556 aa, 61 kDa
• Numéro d’acquisition GenBank: BC006338
• Symbole du gène: CD19
• Identification du gène (NCBI): 930
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C. Éviter toute exposition à la lumière.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).