Anticorps Monoclonal anti-CD19

CD19 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

1D3

N° de cat : CL555-65290

Synonymes

AW495831, Cd19, CD19 antigen

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using CL555-65290

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 555 Anti-Mouse CD19 (CL555-65290, Clone:1D3) or 0.5 ug CoraLite® Plus 555 Rat IgG2a Isotype Control (2A3). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65290 cible CD19 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Mouse CD19 Transfected Cell Line
• Nom complet: CD19 antigen
• Numéro d’acquisition GenBank: BC156767
• Symbole du gène: Cd19
• Identification du gène (NCBI): 12478
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).