Anticorps Monoclonal anti-CD19

CD19 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

SJ25C1

N° de cat : APC-65145

Synonymes

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

---

Galerie de données de validation

• 

  FC experiment of human peripheral blood lymphocytes using APC-65145

  100 ul human peripheral blood were surface stained with FITC Anti-Human CD3 (FITC-65151, Clone: UCHT1) and 5.00 ul APC Anti-Human CD19 (APC-65145, Clone: SJ25C1). Lymphocytes were gated for analysis. Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using APC-65145

  100 ul human peripheral blood were surface stained with FITC Anti-Human CD3 (FITC-65151, Clone: UCHT1) and APC Mouse IgG1 isotype control antibody. Lymphocytes were gated for analysis. Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl(0.25µg) per 10^6 cells in a 100 µl suspension or 5 µl(0.25µg) per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65145 cible CD19 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NALM1 + NALM16 cells
• Nom complet: CD19 molecule
• Masse moléculaire calculée: 556 aa, 61 kDa
• Numéro d’acquisition GenBank: BC006338
• Symbole du gène: CD19
• Identification du gène (NCBI): 930
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C. Éviter toute exposition à la lumière.

Informations générales

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).