Anticorps Recombinant de lapin anti-CD163

CD163 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

GHI/61

N° de cat : 65501-1-RR

Synonymes

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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Galerie de données de validation

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  FC experiment of human PBMCs using 65501-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65501-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65501-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD163 Rabbit Recombinant Antibody (65501-1-RR, Clone:GHI/61) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.2 ug Anti-Human CD163 Mouse Monoclonal Antibody (Clone:GHI/61) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65501-1-RR cible CD163 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Préparation de glycoprotéine à partir de rate de leucémie à cellules velues
• Nom complet: CD163 molecule
• Masse moléculaire calculée: 1156 aa, 125 kDa
• Numéro d’acquisition GenBank: BC051281
• Symbole du gène: CD163
• Identification du gène (NCBI): 9332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).