Anticorps Monoclonal anti-CD16/32

CD16/32 Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2a, lambda

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

93

N° de cat : CL647-65057

Synonymes

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL647-65057

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647 CD16/32 antibody (CL647-65057, Clone: 93 ) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL647-65057

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) and 5 ul CoraLite® Plus 647 Anti-Mouse CD16/32 (CL647-65057, Clone: 93). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL647-65057

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) and CoraLite® Plus 647 conjugated Rat IgG2a isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en IF: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65057 cible CD16/32 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, lambda
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B VH81X Tg et lignée de cellules stromales T220
• Nom complet: Fc receptor, IgG, low affinity IIb
• Numéro d’acquisition GenBank: BC038070
• Symbole du gène: Fcgr2b
• Identification du gène (NCBI): 14130
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 665 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.