Anticorps Monoclonal anti-CD16/32
CD16/32 Monoclonal Antibody for FC
Hôte / Isotype
Rat / IgG2a, lambda
Réactivité testée
souris
Applications
FC
Conjugaison
Atlantic Blue™ Fluorescent Dye
CloneNo.
93
N° de cat : AB-65057
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en cytométrie | splénocytes de souris, |
Dilution recommandée
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
AB-65057 cible CD16/32 dans les applications de FC et montre une réactivité avec des échantillons souris
Réactivité | souris |
Hôte / Isotype | Rat / IgG2a, lambda |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | Lymphocytes B VH81X Tg et lignée de cellules stromales T220 |
Nom complet | Fc receptor, IgG, low affinity IIb |
Numéro d’acquisition GenBank | BC038070 |
Symbole du gène | Fcgr2b |
Identification du gène (NCBI) | 14130 |
Conjugaison | Atlantic Blue™ Fluorescent Dye |
Excitation/Emission maxima wavelengths | 404 nm / 458 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité |
Tampon de stockage | PBS with 0.09% sodium azide. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.
Protocole
Product Specific Protocols | |
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FC protocol for Atlantic Blue™ CD16/32 antibody AB-65057 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |