Anticorps Monoclonal anti-CD16/32

CD16/32 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, lambda

Réactivité testée

souris

Applications

FC

Conjugaison

Atlantic Blue™ Fluorescent Dye

CloneNo.

93

N° de cat : AB-65057

Synonymes

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1



Applications testées

Résultats positifs en cytométriesplénocytes de souris,

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

AB-65057 cible CD16/32 dans les applications de FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG2a, lambda
Clonalité Monoclonal
Type Anticorps
Immunogène Lymphocytes B VH81X Tg et lignée de cellules stromales T220
Nom complet Fc receptor, IgG, low affinity IIb
Numéro d’acquisition GenBankBC038070
Symbole du gène Fcgr2b
Identification du gène (NCBI) 14130
Conjugaison Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.

Protocole

Product Specific Protocols
FC protocol for Atlantic Blue™ CD16/32 antibody AB-65057Download protocol
Standard Protocols
Click here to view our Standard Protocols