Anticorps Monoclonal anti-CD16/32

CD16/32 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, lambda

Réactivité testée

souris et plus (1)

Applications

IF, FC

Conjugaison

Non conjugué

Publications

5

CloneNo.

93

N° de cat : 65057-1-Ig

Synonymes

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Galerie de données de validation

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  FC experiment of BALB/c mouse splenocytes using 65057-1-Ig

  1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16/32 (65057-1-Ig, Clone:93) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed .

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris BALB/c,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below
• FC: See 3 publications below

Informations sur le produit

65057-1-Ig cible CD16/32 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: singe, souris
• Hôte / Isotype: Rat / IgG2a, lambda
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B VH81X Tg et lignée de cellules stromales T220
• Nom complet: Fc receptor, IgG, low affinity IIb
• Numéro d’acquisition GenBank: BC038070
• Symbole du gène: Fcgr2b
• Identification du gène (NCBI): 14130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.

Publications

Species	Application	Title
monkey	FC	Front Microbiol; Response to HIV-1 gp160-carrying recombinant virus HSV-1 and HIV-1 VLP combined vaccine in BALB/c mice; Authors - Beibei Zhang
mouse	IF	Cell Signal; OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization; Authors - Yunyun Li
mouse	IF,FC	J Nanobiotechnology; Bioengineered stem cell membrane functionalized nanoparticles combine anti-inflammatory and antimicrobial properties for sepsis treatment; Authors - Lu Lu
mouse	FC	Stem Cells Dev; Deer antler reserve mesenchyme cell-conditioned medium reduces the destruction of periodontitis in mice; Authors - Hongbing Lin
mouse	IF	BMC Neurosci; KDM4A, involved in the inflammatory and oxidative stress caused by traumatic brain injury-hemorrhagic shock, partly through the regulation of the microglia M1 polarization; Authors - Jimin Cai