Anticorps Monoclonal anti-CD16 / CD32

CD16 / CD32 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2.4G2

N° de cat : CL647-65080

Synonymes

AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using CL647-65080

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with FITC Plus Anti-Mouse CD3 (17A2) and 0.125 ug CoraLite® Plus 647 Anti-Mouse CD16 / CD32 (CL647-65080, Clone:2.4G2) or 0.125 ug CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65080 cible CD16 / CD32 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: Fc receptor, IgG, low affinity IIb
• Numéro d’acquisition GenBank: BC038070
• Symbole du gène: Fcgr2b
• Identification du gène (NCBI): 14130
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898).