Anticorps Monoclonal anti-CD154

CD154 Monoclonal Antibody for FC

Hôte / Isotype

Armenian Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

MR1

N° de cat : FITC-65129

Synonymes

CD154, CD40 ligand, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly 62, Ly62, MR1, T BAM, Tnfsf5, TRAP

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Galerie de données de validation

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  FC experiment of BALB/c mouse CD3+ T cells using FITC-65129

  1X10^6 unstimulated or stimulated (with Cell Stimulation Cocktail, overnight) BALB/c mouse CD3+ T cells were surface stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug FITC Anti-Mouse CD154 (FITC-65129, Clone: MR1). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Stimulated BALB/c mouse CD3+ T cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65129 cible CD154 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Armenian Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD40 ligand
• Numéro d’acquisition GenBank: BC119225
• Symbole du gène: Cd40lg
• Identification du gène (NCBI): 21947
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.1% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.