Anticorps Monoclonal anti-CD154

CD154 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain, primates non humains

Applications

FC

Conjugaison

Non conjugué

CloneNo.

24-31

N° de cat : 65156-1-Ig

Synonymes

CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

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Galerie de données de validation

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  FC experiment of human PBMCs using 65156-1-Ig

  1X10^6 PMA (50 ng/ml) and ionomycin (1 ug/ml)-stimulated human PBMCs (5 hours) were surface stained with 0.2 ug Anti-Human CD154 (65156-1-Ig, Clone: 24-31) and FITC anti-mouse IgG1 Antibody at dilution 1:200 (green), or stained with 0.2 ug isotype control and FITC anti-mouse IgG1 Antibody at dilution 1:200 (black). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65156-1-Ig cible CD154 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains

• Réactivité: Humain, primates non humains
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: protéine de fusion CD154 humaine
• Nom complet: CD40 ligand
• Masse moléculaire calculée: 261 aa, 29 kDa
• Numéro d’acquisition GenBank: BC071754
• Symbole du gène: CD40LG
• Identification du gène (NCBI): 959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.