Anticorps Monoclonal anti-CD146/MCAM

CD146/MCAM Monoclonal Antibody for FC(Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC(Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4D8A9

N° de cat : CL594-66153

Synonymes

CD146, CD146/MCAM, MCAM, MUC18

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Galerie de données de validation

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  FC experiment of HeLa using CL594-66153

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD146/MCAM (CL594-66153, Clone:4D8A9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66153 cible CD146/MCAM dans les applications de FC(Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD146/MCAM Protéine recombinante Ag11855
• Nom complet: melanoma cell adhesion molecule
• Masse moléculaire calculée: 646 aa, 72 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC056418
• Symbole du gène: MCAM
• Identification du gène (NCBI): 4162
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C