Anticorps Monoclonal anti-CD146

CD146 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

canin, Humain, Lapin, souris

Applications

FC

Conjugaison

Non conjugué

Publications

2

CloneNo.

P1H12

N° de cat : 65181-1-Ig

Synonymes

CD146, MCAM, MUC18

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Galerie de données de validation

• 

  FC experiment of HeLa using 65181-1-Ig

  1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human CD146 (65181-1-Ig, Clone:P1H12) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

65181-1-Ig cible CD146 dans les applications de FC et montre une réactivité avec des échantillons canin, Humain, Lapin, souris

• Réactivité: canin, Humain, Lapin, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: cellules ombilicales humaines cultivées
• Nom complet: melanoma cell adhesion molecule
• Masse moléculaire calculée: 646 aa, 72 kDa
• Numéro d’acquisition GenBank: BC056418
• Symbole du gène: MCAM
• Identification du gène (NCBI): 4162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Publications

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi
human	FC	Stem Cell Res Ther; Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models; Authors - Han Li