Anticorps Monoclonal anti-CD14

CD14 Monoclonal Antibody for FC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

FC, ELISA

Conjugaison

Non conjugué

CloneNo.

26IC

N° de cat : 65246-1-Ig

Synonymes

CD14, CD14 molecule

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Galerie de données de validation

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  FC experiment of human PBMCs using 65246-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD14 (65246-1-Ig, Clone:26IC) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000 (red) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65246-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD14 (65246-1-Ig, Clone:26IC) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65246-1-Ig cible CD14 dans les applications de FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD14 molecule
• Masse moléculaire calculée: 375 aa, 40 kDa
• Numéro d’acquisition GenBank: BC010507
• Symbole du gène: CD14
• Identification du gène (NCBI): 929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.