Anticorps Monoclonal anti-CD134

CD134 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

OX-86

N° de cat : CL488-65136

Synonymes

ACT35, CD134, Ly 70, Ox40, Tnfrsf4, Txgp1, TXGP1L

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Galerie de données de validation

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  FC experiment of BALB/c mouse splenocytes using CL488-65136

  1X10^6 Con A-stimulated (3 ug/ml, 2 days) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 5 ul CoraLite® Plus 488 Anti-Mouse CD134 (CL488-65136, Clone: OX-86). Cells were not fixed.

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  FC experiment of BALB/c mouse splenocytes using CL488-65136

  1X10^6 Con A-stimulated (3 ug/ml, 2 days) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-Rat IgG1 isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Con A-stimulated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65136 cible CD134 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: tumor necrosis factor receptor superfamily, member 4
• Numéro d’acquisition GenBank: BC065782
• Symbole du gène: Tnfrsf4
• Identification du gène (NCBI): 22163
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).