Anticorps Monoclonal anti-CD13

CD13 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2D8D11

N° de cat : CL594-66211

Synonymes

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

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Galerie de données de validation

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  IF Staining of mouse kidney using CL594-66211

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CL594-66211 (CD13 antibody) at dilution of 1:100.

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  IF Staining of mouse kidney using CL594-66211

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CL594-66211 (CD13 antibody) at dilution of 1:100.

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  FC experiment of U-937 using CL594-66211

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD13 (CL594-66211, Clone:2D8D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu rénal de souris
• Résultats positifs en cytométrie: cellules U-937

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66211 cible CD13 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD13 Protéine recombinante Ag5976
• Nom complet: alanyl (membrane) aminopeptidase
• Masse moléculaire calculée: 110 kDa
• Numéro d’acquisition GenBank: BC058928
• Symbole du gène: ANPEP
• Identification du gène (NCBI): 290
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174).And this antibody is conjugated with CL594(Ex/Em 593 nm/614 nm).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C