Anticorps Monoclonal anti-CD13

CD13 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain, primates non humains

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

WM15

N° de cat : APC-65186

Synonymes

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

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Galerie de données de validation

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  FC experiment of human peripheral blood granulocytes using APC-65186

  1X10^6 human peripheral blood granulocytes were surface stained with 10.00 ul/test APC Anti-Human CD13 (APC-65186, Clone:WM15) (red) or isotype control antibody (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: granylocytes de sang périphérique humain

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65186 cible CD13 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains

• Réactivité: Humain, primates non humains
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules AML humaines
• Nom complet: alanyl (membrane) aminopeptidase
• Masse moléculaire calculée: 110 kDa
• Numéro d’acquisition GenBank: BC058928
• Symbole du gène: ANPEP
• Identification du gène (NCBI): 290
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à l'allophycocyanine (APC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)