Anticorps Monoclonal anti-CD127 (IL-7Ra)

CD127 (IL-7Ra) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

A7R34

N° de cat : CL488-65093

Synonymes

CD127, CD127 (IL-7Ra), IL 7Ralpha, Il7r, interleukin 7 receptor

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL488-65093

  1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 1 ug CoraLite® Plus 488 Anti-Mouse CD127 (IL-7Ra) (CL488-65093, Clone: A7R34) or 1 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65093 cible CD127 (IL-7Ra) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine de fusion IL-7Ra-IgG1
• Nom complet: interleukin 7 receptor
• Numéro d’acquisition GenBank: BC089571
• Symbole du gène: Il7r
• Identification du gène (NCBI): 16197
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).