Anticorps Monoclonal anti-CD11c

CD11c Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

BU15

N° de cat : 65196-1-Ig

Synonymes

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

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Galerie de données de validation

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  FC experiment of human PBMCs using 65196-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11c (65196-1-Ig, Clone:BU15) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65196-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11c (65196-1-Ig, Clone:BU15) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65196-1-Ig cible CD11c dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules dendritiques de liquide synovial humain
• Nom complet: integrin, alpha X (complement component 3 receptor 4 subunit)
• Masse moléculaire calculée: 1169 aa, 129 kDa
• Numéro d’acquisition GenBank: BC038237
• Symbole du gène: ITGAX
• Identification du gène (NCBI): 3687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).