Anticorps Monoclonal anti-CD11b

CD11b Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

rat

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

Publications

1

CloneNo.

OX42

N° de cat : FITC-65229

Synonymes

Cd11b, integrin alpha M, Itgam

---

Galerie de données de validation

• 

  FC experiment of wistar rat splenocytes using FITC-65229

  1X10^6 Wistar rat splenocytes were surface stained with 0.5 ug FITC Plus Anti-Rat CD11b (FITC-65229, Clone: OX42) or 0.5 ug Control Antibody. Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: Wistar rat splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

FITC-65229 cible CD11b dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Rat peritoneal macrophages
• Nom complet: integrin alpha M
• Masse moléculaire calculée: 127 kDa
• Numéro d’acquisition GenBank: NM_012711
• Symbole du gène: Itgam
• Identification du gène (NCBI): 25021
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
rat	FC	Mol Med Rep; Discovery of microglia gonadotropin‑releasing hormone receptor and its potential role in polycystic ovarian syndrome; Authors - Fan Wang