Anticorps Monoclonal anti-CD11b

CD11b Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

Humain, souris et plus (1)

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications

5

CloneNo.

M1/70

N° de cat : APC-65055

Synonymes

CD11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse bone marrow cells using APC-65055

  1X10^6 C57BL/6 mouse bone marrow cells were surface co-stained with FITC Plus Anti-Mouse Ly-6G/Ly-6C (Gr-1) and 0.1 ug APC Anti-Mouse CD11b (APC-65055, Clone: M1/70) or 0.1 ug APC Rat IgG2b Isotype Control (LTF-2) (APC-65211, Clone: LTF-2). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 5 publications below

Informations sur le produit

APC-65055 cible CD11b dans les applications de FC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T spléniques de souris C57BL/10 et splénocytes C57BL/10 activés par la concanavaline A
• Nom complet: integrin alpha M
• Numéro d’acquisition GenBank: BC156991
• Symbole du gène: Itgam
• Identification du gène (NCBI): 16409
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.1% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
mouse	FC	Adv Sci (Weinh); Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer; Authors - Ting Liang
rat	FC	J Nanobiotechnology; PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer; Authors - Chao Wang
human,mouse	FC	Cell Death Dis; EGFR tyrosine kinase activity and Rab GTPases coordinate EGFR trafficking to regulate macrophage activation in sepsis; Authors - Xuedi Zhang
mouse	FC	Front Bioeng Biotechnol; Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response; Authors - Xinlong Xie
human	FC	Int Immunopharmacol; EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis; Authors - Huiling Zhang