Anticorps Monoclonal anti-CD11B/Integrin Alpha M

CD11B/Integrin Alpha M Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

34

CloneNo.

1C7C2

Avis (1)

N° de cat : 66519-1-Ig

Synonymes

CD11B, CD11B/Integrin Alpha M, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

---

Galerie de données de validation

• 

  WB analysis of THP-1 using 66519-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human bone marrow using 66519-1-Ig

  human bone marrow tissue were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of TF-1 using 66519-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66519-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66519-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66519-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of THP-1 using 66519-1-Ig

  1X10^6 THP-1 cells were stained with 0.20ug CD11B/Integrin alpha M antibody (66519-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules THP-1, cellules TF-1, tissu de moelle osseuse humain
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,
• Résultats positifs en cytométrie: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 18 publications below
• FC: See 1 publications below

Informations sur le produit

66519-1-Ig cible CD11B/Integrin Alpha M dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: integrin, alpha M (complement component 3 receptor 3 subunit)
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: NM_000632
• Symbole du gène: ITGAM
• Identification du gène (NCBI): 3684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
	IF	J Exp Med; TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.; Authors - Dehong Yan
	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
	IF	Apoptosis; ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments; Authors - Qian Yan
	WB	J Neuroinflammation; C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-κB signaling and microglial activation.; Authors - Liu Ye
	IF	Front Immunol; Matrine Inhibits CNS Autoimmunity Through an IFN-β-Dependent Mechanism.; Authors - Yao-Juan Chu
human	IHC,IF	Front Immunol; Redox Regulator GLRX Is Associated With Tumor Immunity in Glioma.; Authors - Yuanhao Chang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsil tissue, HIER pH6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

CD11B/Integrin Alpha M Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66519-1-Ig)