Anticorps Monoclonal anti-CD117
CD117 Monoclonal Antibody for FC
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
bovin, Humain
Applications
FC
Conjugaison
PE Fluorescent Dye
CloneNo.
104D2
N° de cat : PE-65154
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en cytométrie | cellules TF-1 |
Dilution recommandée
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
PE-65154 cible CD117 dans les applications de FC et montre une réactivité avec des échantillons bovin, Humain
Réactivité | bovin, Humain |
Hôte / Isotype | Mouse / IgG1, kappa |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | Cellules mégacaryocytaires MOLM-1 |
Nom complet | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
Masse moléculaire calculée | 976 aa, 110 kDa |
Numéro d’acquisition GenBank | BC071593 |
Symbole du gène | KIT |
Identification du gène (NCBI) | 3815 |
Conjugaison | PE Fluorescent Dye |
Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
Forme | Liquide |
Méthode de purification | L'anticorps purifié est conjugué à la R-phycoérithrine (PE) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille. |
Tampon de stockage | PBS avec azoture de sodium à 0,1 %. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).
Protocole
Product Specific Protocols | |
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FC protocol for PE CD117 antibody PE-65154 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |