Anticorps Monoclonal anti-CD117

CD117 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B8

N° de cat : CL488-65054

Synonymes

Bs, c KIT, CD117, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using CL488-65054

  1X10^6 mouse bone marrow cells were surface stained with APC Anti-Mouse CD45R (B220) (APC-65139, Clone: RA3-6B2) and CoraLite® Plus 488-conjugated Rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD117 (CL488-65054, Clone: 2B8) (right). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65054 cible CD117 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Mastocytes de moelle osseuse dépendants de l'IL-3 de souris
• Nom complet: kit oncogene
• Numéro d’acquisition GenBank: BC052457
• Symbole du gène: Kit
• Identification du gène (NCBI): 16590
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).