Anticorps Monoclonal anti-CD117

CD117 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

bovin, Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

104D2

N° de cat : 65154-1-Ig

Synonymes

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

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Galerie de données de validation

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  FC experiment of TF-1 using 65154-1-Ig

  1X10^6 TF-1 cells were surface stained with 1.5 ug Anti-Human CD117 (65154-1-Ig, Clone:104D2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 1.5 ug Mouse IgG1 Isotype Control Antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules TF-1,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65154-1-Ig cible CD117 dans les applications de FC et montre une réactivité avec des échantillons bovin, Humain

• Réactivité: bovin, Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules mégacaryocytaires MOLM-1
• Nom complet: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Masse moléculaire calculée: 976 aa, 110 kDa
• Numéro d’acquisition GenBank: BC071593
• Symbole du gène: KIT
• Identification du gène (NCBI): 3815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).