Anticorps Monoclonal anti-CD117 (c-Kit)

CD117 (c-Kit) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

ACK2

N° de cat : APC-65094

Synonymes

Bs, c KIT, CD117, CD117 (c-Kit), Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using APC-65094

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.20 ug PE-Anti-Mouse CD11b (PE-65055, clone M1/70) and 0.20 ug APC-Anti-Mouse CD117 (c-Kit) (APC-65094, clone ACK2). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using APC-65094

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.20 ug PE-Anti-Mouse CD11b (PE-65055, clone M1/70) and 0.20 ug APC-Rat IgG2b isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65094 cible CD117 (c-Kit) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Mastocytes dépendant de l'IL-3 de souris
• Nom complet: kit oncogene
• Numéro d’acquisition GenBank: BC052457
• Symbole du gène: Kit
• Identification du gène (NCBI): 16590
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).