Anticorps Monoclonal anti-CD109

CD109 Monoclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2H8G7

N° de cat : 60179-1-Ig

Synonymes

CD109, CD109 antigen, CD109 molecule, CPAMD7, p180, Platelet specific Gov antigen, r150, RP11 525G3.1

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 60179-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60179-1-Ig (CD109 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 60179-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug CD109 antibody (60179-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules Jurkat
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

60179-1-Ig cible CD109 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: CD109 molecule
• Masse moléculaire calculée: 162 kDa
• Poids moléculaire observé: 162 kDa
• Numéro d’acquisition GenBank: BC152996
• Symbole du gène: CD109
• Identification du gène (NCBI): 135228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily. It is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein of approximately 170kd which is found on the cell surface of platelets, activated T-cells, and endothelial cells. CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). CD109 has been identified as part of the TGF-beta receptor system in human keratinocytes. Up regulation of CD109 expression has been observed in several different types of tumor.CD109 is a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the a2-macroglobulin/C3, C4, C5 family of thioester-containing proteins. There're 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. The antibody can recognize all these 4 isoforms.

Publications

Species	Application	Title
human	WB	Clin Oral Implants Res; Characterization of macrophages infiltrating peri-implantitis lesions.; Authors - Tobias Fretwurst
		Drug Dev Res; Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression.; Authors - Junyu Fan
human	IHC	Mol Carcinog; Tandem mass tag (TMT) quantitative protein analysis-based proteomics and parallel reaction monitoring (PRM) validation revealed that MST4 accelerates osteosarcoma proliferation by increasing MRC2 activity; Authors - Yunyan Liu