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Anticorps Monoclonal anti-CD107b / LAMP2

CD107b / LAMP2 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

H4B4

N° de cat : CL750-65053

Synonymes

CD107b, LAMP 2, LAMP2, LAMPB, LGP110

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 750 Anti-Human CD107b / LAMP2 (H4B4 (CL750-65053)

    FC experiment of HeLa using CL750-65053

    1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 750 Anti-Human CD107b (CL750-65053, Clone:H4B4). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

Applications testées

Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-65053 cible CD107b / LAMP2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Cellules adhérentes humaines de sang périphérique
Nom complet lysosomal-associated membrane protein 2
Masse moléculaire calculée 45 kDa
Numéro d’acquisition GenBankBC002965
Symbole du gène LAMP2
Identification du gène (NCBI) 3920
Conjugaison CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).