Anticorps Monoclonal anti-CD107b / LAMP2

CD107b / LAMP2 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H4B4

N° de cat : CL647-65053

Synonymes

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

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Galerie de données de validation

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  IF Staining of HeLa using CL647-65053

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107b antibody (CL647-65053, Clone: H4B4) at dilution of 1:50 and CoraLite®488-conjugated CD107a antibody (CL488-65051, Clone: H4A3, green) at dilution of 1:50.

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  FC experiment of Jurkat using CL647-65053

  1X10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b (CL647-65053, Clone:H4B4) (red) or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65053 cible CD107b / LAMP2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules adhérentes humaines de sang périphérique
• Nom complet: lysosomal-associated membrane protein 2
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: BC002965
• Symbole du gène: LAMP2
• Identification du gène (NCBI): 3920
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C