Anticorps Monoclonal anti-CD107b / LAMP2

CD107b / LAMP2 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG2a

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

FITC Fluorescent Dye

CloneNo.

ABL-93

N° de cat : FITC-65052

Synonymes

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

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Galerie de données de validation

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  FC experiment of NIH/3T3 using FITC-65052

  1X10^6 NIH/3T3 cells were intracellularly stained with 2 ul FITC Anti-Mouse CD107b (FITC-65052, Clone:ABL-93) (red), or 2 ul Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en cytométrie: cellules NIH/3T3

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65052 cible CD107b / LAMP2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glycoprotéines purifiées à partir d'une lignée cellulaire 3T3 d'embryon de souris BALB/c
• Nom complet: lysosomal-associated membrane protein 2
• Numéro d’acquisition GenBank: BC138718
• Symbole du gène: Lamp2
• Identification du gène (NCBI): 16784
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C