Anticorps Monoclonal anti-CD107b / LAMP2

CD107b / LAMP2 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

ABL-93

N° de cat : CL647-65052

Synonymes

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

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Galerie de données de validation

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  IF Staining of NIH/3T3 using CL647-65052

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 647 CD107b antibody (CL647-65052, Clone: ABL-93 ) at dilution of 1:400.

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  FC experiment of NIH/3T3 using CL647-65052

  1X10^6 NIH/3T3 cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD107b (CL647-65052, Clone:ABL-93) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules NIH/3T3,
• Résultats positifs en cytométrie: cellules NIH/3T3

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65052 cible CD107b / LAMP2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glycoprotéines purifiées à partir d'une lignée cellulaire 3T3 d'embryon de souris BALB/c
• Nom complet: lysosomal-associated membrane protein 2
• Numéro d’acquisition GenBank: BC138718
• Symbole du gène: Lamp2
• Identification du gène (NCBI): 16784
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C