Anticorps Monoclonal anti-CD107a / LAMP1

CD107a / LAMP1 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

H4A3

N° de cat : CL405-65051

Synonymes

CD107a, LAMP 1, LAMP1, LAMPA, LGP120

---

Galerie de données de validation

• 

  FC experiment of HeLa using CL405-65051

  1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD107a / LAMP1 (CL405-65051, Clone:H4A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using CL405-65051

  1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD107a / LAMP1 (CL405-65051, Clone:H4A3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-65051 cible CD107a / LAMP1 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules adhérentes humaines de sang périphérique
• Nom complet: lysosomal-associated membrane protein 1
• Numéro d’acquisition GenBank: BC006345
• Symbole du gène: LAMP1
• Identification du gène (NCBI): 3916
• Conjugaison: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).