Anticorps Monoclonal anti-CD107a / LAMP1
CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF
Hôte / Isotype
Rat / IgG2a, kappa
Réactivité testée
souris
Applications
IF, FC (Intra)
Conjugaison
APC Fluorescent Dye
CloneNo.
1D4B
N° de cat : APC-65050
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en IF | cellules NIH/3T3, |
Résultats positifs en cytométrie | cellules NIH/3T3 |
Dilution recommandée
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
IF | See 1 publications below |
Informations sur le produit
APC-65050 cible CD107a / LAMP1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons souris
Réactivité | souris |
Réactivité citée | souris |
Hôte / Isotype | Rat / IgG2a, kappa |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | Fraction de la membrane plasmique de la lignée cellulaire NIH/3T3 d'embryon de souris |
Nom complet | lysosomal-associated membrane protein 1 |
Numéro d’acquisition GenBank | BC006785 |
Symbole du gène | Lamp1 |
Identification du gène (NCBI) | 16783 |
Conjugaison | APC Fluorescent Dye |
Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité |
Tampon de stockage | PBS avec azoture de sodium à 0,1 % et un agent stabilisant. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocole
Product Specific Protocols | |
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IF protocol for APC CD107a / LAMP1 antibody APC-65050 | Download protocol |
FC protocol for APC CD107a / LAMP1 antibody APC-65050 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Pharmacol Res RHBDF1 deficiency suppresses melanoma glycolysis and enhances efficacy of immunotherapy by facilitating glucose-6-phosphate isomerase degradation via TRIM32 |