Anticorps Monoclonal anti-CD103

CD103 Monoclonal Antibody for FC

Hôte / Isotype

Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2E7

N° de cat : CL647-65047

Synonymes

A530055J10, alpha E1, alpha M290, aM290, CD103, Itgae

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Galerie de données de validation

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  FC experiment of BALB/c mouse splenocytes using CL647-65047

  1X10^6 BALB/c mouse splenocytes were surface stained with 5 ul CoraLite®488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated Armenian Hamster IgG isotype control (left) or 5 ul CoraLite® Plus 647 Anti-Mouse CD103 (CL647-65047, Clone:2E7) (right). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65047 cible CD103 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes intraépithéliaux (LIE) intestinaux de souris C57BL/6J
• Nom complet: integrin alpha E, epithelial-associated
• Numéro d’acquisition GenBank: BC150690
• Symbole du gène: Itgae
• Identification du gène (NCBI): 16407
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).