Anticorps Monoclonal anti-CCT7

• Phare
• Validé par KD/KO

CCT7 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2C1G1

N° de cat : 67540-1-Ig

Synonymes

CCT ETA, CCT7, Ccth, HIV 1 Nef interacting protein, Nip7 1, TCP 1 eta

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Galerie de données de validation

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  WB analysis using 67540-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HEK-293 using 67540-1-Ig

  WB result of CCT7 antibody (67540-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT7 transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 67540-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 67540-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U2OS using 67540-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IF: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67540-1-Ig cible CCT7 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCT7 Protéine recombinante Ag9502
• Nom complet: chaperonin containing TCP1, subunit 7 (eta)
• Masse moléculaire calculée: 543 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC019296
• Symbole du gène: CCT7
• Identification du gène (NCBI): 10574
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

Publications

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita