Anticorps Polyclonal de lapin anti-CCR7

CCR7 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 25898-1-AP

Synonymes

BLR2, C C chemokine receptor type 7, C C CKR 7, CC CKR 7, CCR 7, CCR7, CD197, CDw197, CMKBR7, EBI1, EVI1, MIP 3 beta receptor

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Galerie de données de validation

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  WB analysis using 25898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25898-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 25898-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25898-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 25898-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25898-1-AP (CCR7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25898-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25898-1-AP (CCR7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules C6, cellules K-562
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Informations sur le produit

25898-1-AP cible CCR7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCR7 Protéine recombinante Ag22941
• Nom complet: chemokine (C-C motif) receptor 7
• Masse moléculaire calculée: 378 aa, 43 kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: BC035343
• Symbole du gène: CCR7
• Identification du gène (NCBI): 1236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C-C chemokine receptor type 7 (CCR7), which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probably acts as a mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

Publications

Species	Application	Title
rat	IF	Bioact Mater; Two-stage degradation and novel functional endothelium characteristics of a 3-D printed bioresorbable scaffold.; Authors - Tieying Yin
mouse	IHC	Virulence; Intranasal bovine β-defensin-5 enhances antituberculosis immunity in a mouse model by a novel protein-based respiratory mucosal vaccine.; Authors - Zhengmin Liang
rat	IHC	Front Cell Dev Biol; Potential Mechanisms of the Impact of Hepatocyte Growth Factor Gene-Modified Tendon Stem Cells on Tendon Healing.; Authors - Mingzhao Zhang
rat	IF	Stem Cell Res Ther; Tendon stem cell-derived exosomes regulate inflammation and promote the high-quality healing of injured tendon.; Authors - Mingzhao Zhang
human	IHC	Front Mol Biosci; CCR7 Has Potential to Be a Prognosis Marker for Cervical Squamous Cell Carcinoma and an Index for Tumor Microenvironment Change.; Authors - Wei-Jie Tian
human	WB	Front Pharmacol; MicroRNA-532-3p Suppresses Malignant Behaviors of Tongue Squamous Cell Carcinoma via Regulating CCR7.; Authors - Cuijuan Feng